Epitopes described in "Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product."

Article Authors:S Usuda; H Okamoto; H Iwanari; K Baba; F Tsuda; Y Miyakawa; M Mayumi
Article Title:Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product.
Reference Detail
Reference ID:1003373
Abstract:An ELISA was developed for serological determination of the six genotypes of hepatitis B virus (HBV) designated A, B, C, D, E, and F. Monoclonal antibodies were raised against genotype-specific epitopes in the preS2-region product, and labeled with horseradish peroxidase. Hepatitis B surface antigen (HBsAg) in sera was captured by immobilized antibodies against the common determinant, and evaluated for reactivity with genotype-specific monoclonal antibodies labeled with the enzyme. Serological genotyping was in complete accord with genotypes determined by S-gene sequences in a panel of 68 sera containing HBV/HBsAg of different genotypes. Of 514 sera with HBsAg from Japan, 507 (98.6%) were genotyped serologically: genotype A was identified in 24 (4.7%); B in 196 (38.1%); C in 282 (54.9%); D in 2 (0.4%); and F in 3 (0.6%). There were no sera containing HBV of genotype E. Likewise, 425 of 446 (95.3%) sera with HBsAg from Brazil, China, India, Indonesia, Kenya, Korea, Nepal, Papua New Guinea, the Philippines, and Thailand were classified into A (25.6%), B (24.2%), C (33.9%), and D (11.7%) genotypes; there were no sera with HBsAg of genotype E or F among them. Some sera unclassifiable by ELISA revealed mixed infection with HBV of distinct genotypes, or contained HBsAg deprived of genotype-specific epitopes by point mutations. The ELISA would be useful for large-scale surveys, because it allows serological detection of HBV genotypes without sequencing nucleotides.
Affiliations:Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan.
Reference Type:Literature
PubMed ID:10403681
Journal:J Virol Methods
Journal Volume:80
Article Pages:97-112
Journal ISSN:0166-0934
Article Chemical List:Antibodies, Monoclonal;Epitopes, B-Lymphocyte;Hepatitis B Antibodies;Hepatitis B Surface Antigens;Protein Precursors;presurface protein 2, hepatitis B surface antigen
Article MeSH List:Amino Acid Sequence; Antibodies, Monoclonal(immunology ); Antibody Specificity; Enzyme-Linked Immunosorbent Assay(methods ); Epitopes, B-Lymphocyte(immunology ); Genotype; Hepatitis B(blood; immunology; virology ); Hepatitis B Antibodies(immunology ); Hepatitis B Surface Antigens(immunology ); Hepatitis B virus(classification; genetics; immunology ); Humans; Japan; Molecular Sequence Data; Protein Precursors(immunology )
Curation Last Updated:2014-10-03 20:25:02