Epitopes described in "Characterization of IgG monoclonal anti-cardiolipin/anti-beta2GP1 antibodies from two patients with antiphospholipid syndrome reveals three species of antibodies."

Reference
Article Authors:M Zhu; T Olee; D T Le; R A Roubey; B H Hahn; V L Woods Jr; P P Chen
Article Title:Characterization of IgG monoclonal anti-cardiolipin/anti-beta2GP1 antibodies from two patients with antiphospholipid syndrome reveals three species of antibodies.
Reference Detail
Reference ID:1016227
Abstract:Antiphospholipid antibodies (aPL), including antibodies detected in anti-cardiolipin (aCL) enzyme-linked immunosorbent assays and in lupus anticoagulant (LA) tests, are strongly associated with recurrent thrombosis and recurrent fetal loss, i.e. the antiphospholipid syndrome (APS). Although recent studies suggest that most APS-associated aCL are directed against the phospholipid (PL)-binding plasma protein beta2-glycoprotein 1 (beta2GP1), the precise nature of aCL binding specificities remains controversial. To address the issue of aCL specificity we generated five new monoclonal IgG aCL from two patients with APS. Characterization of these five aCL, as well as two previously published IgG aCL, revealed three patterns of reactivity: (1) four antibodies reacted strongly with human beta2GP1-cardiolipin (CL) complexes and weakly with human beta2GP1 alone; (2) two antibodies recognized bovine beta2GP1, but not human beta2GP1; (3) one antibody reacted with complexes of human beta2GP1 and CL, but not with human beta2GP1 alone. Only one monoclonal displayed weak LA activity. These patient-derived IgG monoclonal antibodies, and additional ones to be generated, may help define varying species of antibodies detected in aCL assays and identify the specific antibodies that may be pathogenic.
Affiliations:Department of Medicine, Division of Rheumatology, University of California at Los Angeles, Los Angeles, California, USA.
Date:1999
Reference Type:Literature
PubMed ID:10233371
Journal:Br J Haematol
Journal Volume:105
Article Pages:102-9
Journal ISSN:0007-1048
Article Chemical List:Antibodies;Antibodies, Anticardiolipin;Anticoagulants;Glycoproteins;Immunoglobulin G;Lupus Coagulation Inhibitor;beta 2-Glycoprotein I
Article MeSH List:Adolescent; Adult; Antibodies(immunology); Antibodies, Anticardiolipin(immunology); Anticoagulants(immunology); Antiphospholipid Syndrome(immunology); Female; Glycoproteins(immunology); Humans; Immunoglobulin G(immunology); Lupus Coagulation Inhibitor(immunology); Male; beta 2-Glycoprotein I
Curation Last Updated:2014-04-11 20:11:03