Epitopes described in "Sequence analysis of Pasteurella multocida major outer membrane protein (OmpH) and application of synthetic peptides in vaccination of chickens against homologous strain challenge."

Reference
Article Authors:Y Luo; Q Zeng; J R Glisson; M W Jackwood; I H Cheng; C Wang
Article Title:Sequence analysis of Pasteurella multocida major outer membrane protein (OmpH) and application of synthetic peptides in vaccination of chickens against homologous strain challenge.
Reference Detail
Reference ID:1006492
Abstract:Pasteurella multocida major outer membrane protein (OmpH) has been previously characterized as a porin. The native OmpH from strain X-73 (serotype 1) but not recombinant protein from Escherichia coli induced homologous protection in chickens. In this study OmpH sequences from 15 P. multocida serotypes as well as the CU vaccine strain were compared by sequence alignment and revealed high homology, with major variations confined to two discrete regions which were correspondingly predicted as two largest external loops. Secondary structures of OmpHs were predicted by sequence alignment of OmpHs with well defined porins and analyses of amphiphilicity, hydrophobic moment and antigenic index plots. Several synthetic peptides derived from predicted loop 2 and loop 5 of X-73 OmpH were synthesized as vaccine candidates. Vaccination studies in chickens showed that the cyclic synthetic peptide (Cyclic-L2) mimicking the predicted loop 2 induced 70% protection in chickens against strain X-73 challenge. This is the first report that a synthetic peptide mimicking the conformational epitopes of a native protein provide practical protection in target animal against bacterial infection.
Affiliations:Department of Avian Medicine, College of Veterinary Medicine, The University of Georgia, Athens 30602, USA. jglisson@u-ga.cc.uga.edu
Date:1999
Reference Type:Literature
PubMed ID:10067687
Journal:Vaccine
Journal Volume:17
Article Pages:821-31
Journal ISSN:0264-410X
Article Chemical List:Bacterial Outer Membrane Proteins;Bacterial Vaccines;Peptide Fragments;Vaccines, Synthetic;ompH protein, bacteria
Article MeSH List:Amino Acid Sequence; Animals; Bacterial Outer Membrane Proteins(genetics; immunology ); Bacterial Vaccines(immunology; therapeutic use ); Chickens; Molecular Sequence Data; Pasteurella Infections(immunology; prevention & control; veterinary ); Pasteurella multocida(immunology ); Peptide Fragments(genetics; immunology ); Poultry Diseases(immunology; prevention & control ); Sequence Homology, Amino Acid; Serotyping; Vaccines, Synthetic(genetics; immunology; therapeutic use )
Curation Last Updated:2014-07-16 21:20:19